A bamboo whisk rests in a black bowl of frothy green matcha tea.

Why Matcha Has So Much L-Theanine (And Why It Matters)

Written by: Mecene Research Team

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Published

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Time to read 7 min

Matcha green tea is a powdered green tea made from whole leaves and is especially rich in L-theanine. This naturally occurring amino acid helps influence how the brain responds to caffeine, stress, and relaxation. Matcha stands out from other green teas because shade-grown cultivation and whole-leaf consumption deliver more L-theanine per cup. This article explains how L-theanine in matcha works, its benefits, and why matcha is a top natural source.

What Is L-Theanine And What Does It Do?

L-theanine is a non-protein amino acid found naturally in tea leaves from the tea plant Camellia sinensis. Unlike amino acids that form proteins, L-theanine is studied for how it may influence relaxation and attention.


Research suggests L-theanine can cross the blood-brain barrier, which is one reason it is discussed in the context of mood and focus. Some studies also link L-theanine with increased alpha brain wave activity, which is associated with calm alertness.


L-theanine is often described as promoting relaxation without heavy drowsiness. In matcha green tea, L-theanine naturally pairs with caffeine. That pairing is one reason matcha is commonly described as “calm energy” rather than the sharp stimulation some people feel from coffee.

A glass cup of green tea sits on a saucer, accompanied by loose leaf tea in a wooden bowl, matcha powder in a small dish, a lemon wedge, and fresh tea leaves.

How Much L-Theanine Is in Matcha?

Matcha typically contains 15–40 mg of L-theanine per serving, depending on matcha powder quality, harvest timing, and the amount of powder used. Because matcha is consumed whole rather than steeped and discarded, the full amino acid profile ends up in the drink.


Because the whole leaf is consumed, the drink can contain more of the leaf’s naturally occurring L-theanine than a typical steep-and-strain cup. Shading is linked with higher amino acids (including theanine) and lower catechin levels, which can make matcha taste smoother and less bitter.


Below is a general comparison of L-theanine across common teas. Values vary by preparation, water temperature, steep time, and tea quality.

Tea type

Typical L-theanine level (general)

Why it differs

Matcha

Higher

Shade-grown leaves and whole-leaf consumption

Green tea

Moderate

Varies by cultivar, harvest, and steeping

Black tea

Lower

Processing and oxidation shift the tea’s compound profile

L-Theanine in matcha.

Why Matcha Has More L-Theanine Than Other Teas

Matcha contains more L-theanine than many other green teas due to its growing and processing methods. Shade-growing slows photosynthesis and increases amino acid retention, including L-theanine, while reducing harsh flavors.

Matcha is made from whole, stone-ground tea leaves called tencha, which are fully consumed. Brewed tea leaves are removed after steeping, limiting amino acid delivery. High-grade Japanese matcha is also harvested earlier, when L-theanine levels are naturally higher.

Caffeine and L-Theanine in Matcha: Why the Combo Works

L-theanine and caffeine work together in matcha to create sustained focus without excessive stimulation. While coffee delivers more caffeine more quickly, many people report that matcha feels steadier than coffee, which may be due to L-theanine paired with caffeine.


Caffeine supports alertness and reaction speed, while L-theanine may support calm focus and smoother physiological stress responses. Together, this pairing is associated with improved reaction time and mental clarity compared to caffeine alone in some studies.

"Caffeine supports alertness and reaction speed, while L-theanine may support calm focus and smoother physiological stress responses."

What Makes the Combo Unique?

Matcha naturally delivers theanine and caffeine in the same drink, without isolated stimulants or dietary supplements. A serving of matcha, typically 1–2 grams, provides a moderate amount of caffeine alongside L-theanine.

Instead of promising “no jitters,” research suggests that L-theanine may affect how caffeine feels, especially when matcha replaces high-dose coffee beverages. Individual response varies.

Scientific Benefits of the Pair

Research suggests that combining L-theanine and caffeine may improve reaction time and attention compared to a placebo group. Some studies show better task performance than caffeine alone, particularly during a defined practice period.

Observed positive effects depend on dose, baseline stress, and individual sensitivity; therefore, results are described as supportive rather than guaranteed.

Iced matcha latte in a clear plastic cup shows a vibrant green layer at the bottom, a creamy tan middle, and a dark brown coffee top with ice cubes.

What Are the Health Benefits of L-Theanine in Matcha?

L-theanine in matcha is associated with support for relaxation during stress, mood, and mental performance. These benefits support daily health, not medical treatment.

Enhances Focus and Productivity

L-theanine may support sustained focus and clearer thinking by promoting a relaxed but alert brain state. For practical use, matcha can be a good option before deep work, reading, or long meetings that require sustained attention.

Reduces Stress and Promotes Calm

L-theanine is often studied for how it may support relaxation during stress. Many people describe the effect as a calmer baseline rather than sedation.


This is best framed as support for everyday stress, not a treatment for a medical condition.

May Support Better Sleep (Especially in the Evening)

L-theanine is studied for relaxation, which can be helpful when winding down at night. Matcha still contains caffeine, so evening use is usually a better fit with a decaf matcha or a caffeine-free routine.


For evening routines, consider caffeine-free habits such as herbal tea, warm milk, or a decaf matcha product made specifically for nighttime use.

May Help Lower Blood Pressure During Stress

Some research suggests L-theanine may support a healthy blood pressure response during acute mental stress in certain adults. This is not a treatment for high blood pressure, and it does not replace medical care.

Supports a Healthy Immune Response

Every day stress can affect overall wellness, including immune function. Early research on L-theanine and immune-related measurements is still developing, so it is best framed as general wellness support, not a guaranteed outcome.

A person drinks green matcha from a speckled ceramic cup.

How L-Theanine Affects the Way Matcha Tastes

L-theanine contributes to matcha’s rich umami taste and smooth mouthfeel. Higher amino acid content is one reason high-quality matcha often tastes creamy and less bitter.


Matcha also contains catechins, which can taste astringent. When the amino acid and catechin balance shifts toward more amino acids, matcha tends to taste smoother.

Ceremonial-style matcha is often made from younger leaves and is commonly milder and more rounded. Culinary matcha is usually more bitter and is better suited for baking and lattes, where other flavors are added.

When Should You Drink Matcha for The Best L-Theanine Effects?

Matcha timing affects how L-theanine and caffeine interact.

  • Morning: Calm alertness and steady energy

  • Before work or study: Sustained focus and productivity

  • Mid-afternoon: Gentle lift with less crash

  • Evening (decaf): Relaxation without stimulation

How to Choose High L-Theanine Matcha

Choose Japanese matcha that is ceremonial-grade, shade-grown, and bright green. Younger leaves and proper processing help preserve L-theanine and flavor.

Quick Quality Checklist

A white cup of matcha latte with a bowl of matcha powder and whisk in the background.

Why Matcha Is the Best Way to Get L-Theanine

Matcha is one of the most convenient tea-based sources of L-theanine because the entire tea leaf is consumed rather than steeped and discarded. Unlike standalone L-theanine supplements, matcha provides L-theanine alongside naturally occurring caffeine and tea polyphenols.


This whole-leaf approach makes matcha a practical daily option for supporting calm focus, balanced mood, and overall well-being, without relying on isolated ingredients.

Ready to upgrade the daily cup? Explore Mécène Market’s premium ceremonial-grade matcha and enjoy a cup that pairs well with calm, focused mornings.

Frequently Asked Questions

How much L-theanine is in a cup of matcha?

A cup of matcha typically contains about 15–40 mg of L-theanine, depending on grade, quality, and the amount of powder used.

Is matcha higher in L-theanine than green tea?

Yes, matcha often provides more L-theanine than brewed green tea because it is shade-grown and the whole leaf is consumed.

Can I get L-theanine from other foods?

L-theanine is found mainly in tea, with trace amounts reported in a small number of mushrooms.

Does L-theanine in matcha help with focus?

L-theanine in matcha may support calm focus, especially when paired with matcha’s natural caffeine.

What’s the best time to drink matcha for calm energy?

Morning or early afternoon is often a good fit, especially for people sensitive to caffeine later in the day.

REFERENCES

  1. Harvard Health Publishing. (n.d.). Matcha: A look at possible health benefits. https://www.health.harvard.edu/staying-healthy/matcha-a-look-at-possible-health-benefits

  2. Hidese S, Ogawa S, Ota M, Ishida I, Yasukawa Z, Ozeki M, Kunugi H. Effects of L-Theanine Administration on Stress-Related Symptoms and Cognitive Functions in Healthy Adults: A Randomized Controlled Trial. Nutrients. 2019 Oct 3;11(10):2362. doi: 10.3390/nu11102362. PMID: 31623400; PMCID: PMC6836118.

  3. Li MY, Liu HY, Wu DT, Kenaan A, Geng F, Li HB, Gunaratne A, Li H, Gan RY. L-Theanine: A Unique Functional Amino Acid in Tea (Camellia sinensis L.) With Multiple Health Benefits and Food Applications. Front Nutr. 2022 Apr 4;9:853846. doi: 10.3389/fnut.2022.853846. PMID: 35445053; PMCID: PMC9014247.

  4. Owen GN, Parnell H, De Bruin EA, Rycroft JA. The combined effects of L-theanine and caffeine on cognitive performance and mood. Nutr Neurosci. 2008 Aug;11(4):193-8. doi: 10.1179/147683008X301513. PMID: 18681988.

  5. Uchida, K., Meno, K., Korenaga, T., Liu, S., Suzuki, H., Baba, Y., … Kobayashi, M. (2024). Effect of matcha green tea on cognitive functions and sleep quality in older adults with cognitive decline: A randomized controlled study over 12 months. PLOS ONE, 19(8), e0309287. https://doi.org/10.1371/journal.pone.0309287

  6. Unno T., et al. Health Benefits and Chemical Composition of Matcha Green Tea: A Review. Molecules (2020). Found up to 44.65 mg/g of L-theanine in matcha tea samples, supporting typical serving estimates in the 15–40 mg range.

Medically Reviewed By

Dr. James Pendleton

Dr. James Pendleton is a primary care physician specializing in a naturopathic approach to family medicine. He has nurtured a family practice in Seattle, directed a VIP medical center in Abu Dhabi, published several books and scientific articles, and designed innovative nutritional supplements for manufacturers worldwide.