Matcha powder on a black bowl.

How Much L-Theanine Is in Green Tea? A Calm-Boosting Breakdown

Written by: Mecene Research Team

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Published

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Time to read 8 min

If green tea helps you feel calm yet alert, L-theanine is a big reason why. That experience often leads people to ask how much L-theanine green tea actually provides in a typical cup. This calming compound is found mostly in tea leaves, especially green tea and matcha.


Some cups contain only a small amount, while others contain much more. Matcha and shade-grown teas often land near the top of the range. This article explains what drives those differences and how to apply them to daily habits.

This content is for educational purposes only and is not intended as medical advice.

What Is L-Theanine and Why Is It Found in Tea?

L-theanine is a unique amino acid found primarily in the Camellia sinensis plant, which is used to produce green, black, oolong, and white tea. This is the same tea plant used to make green, black, oolong, and white tea. Research says that unlike many other amino acids, this compound does not contribute to protein synthesis in the body.

Instead, amino acid L-theanine influences activity in the central nervous system. In food chemistry, researchers describe it as a beneficial compound that can promote relaxation and support steady attention. Green tea contains this compound because it forms naturally in tea leaves as the plant grows and processes nutrients.

A clear glass cup filled with green tea that contains L-theanine, often makes people wonder how much L-theanine in green tea they can get.

How Much L-Theanine Is in Green Tea?

L-theanine levels vary by tea type because growing methods and processing affect the compounds in the tea leaf.


The table below shows typical ranges seen across tea samples, including commercial tea samples and common styles.

Tea Type

Avg. L-Theanine (mg)

Serving Size

Powdered green tea (Matcha)

20–40 mg L-theanine

1 gram powder (½ teaspoon)

Gyokuro green tea

12–20 mg

1 cup of tea (250 milliliters)

Sencha

5–8 mg

1 cup of tea (250 milliliters)

Black tea

2–5 mg

1 cup of tea (250 milliliters)

White teas and oolong teas

3–6 mg

1 cup of tea (250 milliliters)


Matcha tends to deliver the most because the full tea leaf is consumed. In contrast, many infusions prepared from loose leaves or a tea bag leave some amino acids behind in the discarded leaves.

L-Theanine Content by Tea Type (MG per Serving)

The answer depends on the tea type, the growing method, and the infusion method. Many cups of green tea contain 5 to 20 milligrams of L-theanine, while some teas can reach higher levels. That means the question of how much L-theanine can change from one tea bag to another, even within the same brand.

"L-theanine levels vary by tea type because growing methods and processing affect the compounds in the tea leaf."

What Affects the Amount of L-Theanine in Green Tea?

Several factors influence the average theanine concentration in a cup of green tea. These differences help explain why tea samples vary in taste, caffeine concentration, and calming feel.

Tea Growing Method

Shade-growing increases L-theanine by reducing direct sunlight. Less sunlight slows the conversion of amino acids into catechins, which can reduce bitterness. That is one reason shade-grown styles, including some Japanese green tea types, often show higher theanine content than sun-grown teas.

Leaf Age and Harvest

Younger leaves often contain more L-theanine and fewer harsh flavor notes. First-flush teas and early-harvest leaves can be richer in amino acids than later-flush teas. This pattern appears across many green and white teas, especially in unfermented teas.

How You Brew Your Tea

Tea preparation typically shapes what moves from the tea leaf into the liquid. Lower water temperatures and shorter steep times help preserve amino acids. Hotter water and longer steeping can raise bitterness and shift the balance between L-theanine and caffeine.

Rows of lush green tea plants are growing in a field under a dark mesh shade cover.

Which Green Teas Have the Most L-Theanine?

Some green teas rank higher for L-theanine content due to shade-grown cultivation and careful processing. Note that the exact number still varies by farm, batch, and storage.


The following options commonly contain more L-theanine than standard bagged tea:


Many of these teas are unfermented, while fermented teas such as pu'erh usually taste deeper and earthier. Black and oolong teas are also compared for differences in caffeine levels and overall flavor balance.

How L-Theanine Works in Your Body

After tea consumption, L-theanine can influence the brain in ways linked with calm attention. This beneficial compound crosses the blood-brain barrier and interacts with neurotransmitters involved in mood and focus. The effects of L-theanine can feel subtle, yet noticeable in a warm cup taken during a busy day.

Supports Calm Brain Waves

L-theanine may be better as a tea for gentle, lifestyle-based relaxation, while supplements may suit those who prefer a consistent, higher dose for targeted sleep support. Tea offers a lower amount of L-theanine along with warmth and routine, which can help promote relaxation in the evening. Supplements provide precise dosing and may be more suitable for people seeking stronger or more predictable effects.


Source

Typical Amount

Additional Benefits

Green tea

10–40 mg

Ritual, hydration

Matcha

30–70 mg

Focus, antioxidants

Supplements

100–400 mg

Precise L-Theanine dosage


Some products use a L-Theanine mixture alongside magnesium or valerian root. Choosing between tea and supplementation often depends on lifestyle and sensitivity.

Pairs Well With Caffeine

The combination of L-theanine and caffeine is often described as smoother than caffeine alone. This pairing, sometimes written as L-theanine and caffeine, can help counteract the overstimulation that can occur with caffeine consumption. Green tea often has a slightly higher caffeine concentration than herbal teas, yet the amino acids help balance the experience.

A person with long brown hair wearing a light blue shirt and earrings drinks from a light green mug.

How to Get More L-Theanine From Your Tea

Small changes in tea preparation can raise L-theanine content in the cup. These steps focus on preserving amino acids while maintaining a smooth flavor. The goal is to pull more L-theanine from tea leaves without pushing bitterness too far.

Brew Smarter

Using cooler water and shorter steep times can protect amino acids. Aim for water between 160 and 175 degrees Fahrenheit, and steep for 1 to 2 minutes. This approach can promote relaxation and reduce bitter taste.

Choose High-L-Theanine Teas

Shade-grown teas, ceremonial-grade matcha, and gyokuro often have higher levels. First flush teas can also contain more amino acids than later harvests. Commercial tea samples can vary widely, so labels and sourcing notes can help.

Build a Daily Ritual

A consistent routine can make L-theanine intake more predictable. Many tea drinkers enjoy a cup of tea in the morning or before a focused work session. Pairing tea with food can help moderate the feel of caffeine concentration.

A person pours green tea from a brown teapot into two clear glasses, with bowls of matcha and loose leaf tea nearby.

L-Theanine in Green Tea vs. Supplements

L-theanine supplements provide isolated doses, often labeled 100-200 milligrams. Green tea extracts and brewed tea usually provide smaller amounts, yet also contain polyphenols and other amino acids. This difference matters for people who prefer a food-first approach.


Tea fits easily into daily habits and supports human health broadly. Supplements provide a fixed dose, while tea offers a range shaped by tea type and brewing methods. For a gentle approach, green tea can be a practical option.

Are There Any Risks or Side Effects?

L-theanine from tea is generally safe for most healthy adults in typical amounts. Sensitivity usually relates to caffeine content rather than the amino acid itself. Some green teas can have a mean caffeine concentration that feels strong, especially when matcha is used.


People who are pregnant or managing medications may want guidance from a qualified professional before raising caffeine consumption or supplement use. Some research explores sleep quality and blood pressure in relation to tea, but tea is not a treatment for any condition. Herbal teas that do not come from Camellia sinensis do not contain L-theanine.

Sip Your Way to Gentle Focus

Green tea contains L-theanine, a unique amino acid that can promote relaxation while supporting alertness. For higher theanine content, shade-grown teas such as matcha and gyokuro usually land at the top, especially when tea brewing temperatures stay on the lower side.


For a simple next step, choose one tea type and keep brewing consistently. Use water at 160 to 175 degrees Fahrenheit and steep for one to two minutes. Then adjust based on caffeine content so the cup feels steady from day to day.

Frequently Asked Questions

How much L-theanine is in a cup of green tea?

A typical cup of green tea contains about 5 to 20 milligrams of L-theanine, depending on the tea type and how it is prepared.

Which green tea has the highest L-theanine content?

Powdered green tea and gyokuro green tea often have the highest L-theanine content because shade-growing supports higher amino acid levels.

Is matcha the best source of L-theanine?

Yes, matcha is often the best tea-based source because the whole tea leaf is consumed, not discarded after brewing.

Can you get enough L-theanine from drinking tea?

Yes, many tea drinkers get meaningful amounts through regular tea consumption, especially with certain teas such as matcha or gyokuro.

Does brewing time affect L-theanine levels in tea?

Yes, shorter steep times and lower temperatures can help preserve amino acids while reducing bitterness.

REFERENCES

  1. Ho, L. T., & Kuhnert, N. (2022). L-theanine in tea and its physiology in humans: A comprehensive review. Frontiers in Nutrition. https://pmc.ncbi.nlm.nih.gov/articles/PMC9014247/

  2. Kaur, C., Kapoor, H. C., & Sikka, S. (2021). Tea phytochemicals and health: A detailed review. Plant Foods for Human Nutrition. https://pmc.ncbi.nlm.nih.gov/articles/PMC7647716/

  3. Lee, J., Lee, J., & Kim, T. (2022). Metabolic pathways and physiological effects of L-theanine: Insights from tea plant studies. Journal of Food Science and Technology. https://pmc.ncbi.nlm.nih.gov/articles/PMC9017334/

  4. Liu, Z., Huang, C., & Li, J. (2022). Bioactive compounds in tea: Chemical composition and potential health effects. Journal of Food Composition and Analysis. https://pmc.ncbi.nlm.nih.gov/articles/PMC9696345/

  5. Nobre, A. C., Rao, A., & Owen, G. N. (2008). L-theanine, a natural constituent in tea, and its effect on mental state. Asia Pacific Journal of Clinical Nutrition, 17(Suppl 1), 167-168. https://pubmed.ncbi.nlm.nih.gov/18296328/

  6. Sokary, S., Al-Asmakh, M., Zakaria, Z., & Bawadi, H. (2022). The therapeutic potential of matcha tea: A critical review on human and animal studies. Current research in food science, 6, 100396. https://doi.org/10.1016/j.crfs.2022.11.015

  7. Zhao, H., Dong, J., Lu, J., & Chen, S. (2019). A review of tea polyphenols and their benefits to human health. Food & Nutrition Research. https://pmc.ncbi.nlm.nih.gov/articles/PMC11643846/

Medically Reviewed By

Dr. James Pendleton

Dr. James Pendleton is a primary care physician specializing in a naturopathic approach to family medicine. He has nurtured a family practice in Seattle, directed a VIP medical center in Abu Dhabi, published several books and scientific articles, and designed innovative nutritional supplements for manufacturers worldwide.